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e Mean read coverage per genomic base.
Structural variation in plasmids was identified by plotting the mean read coverage for each 1,000 bp of the reference sequence divided by the mean read coverage across the whole reference sequence, capped to a maximum of one.
One method for picking the highest covered transcript from a locus from Oases assemblies includes choosing the isoform with the highest geometric mean read coverage across nodes [ 9].
The mean read coverage of our event sequences ranges from 0.1285 to 2135, with a median of 33.15, a mean of 123.3 and a standard deviation of 245.7.
After generating reads, 74 simulated PTDs, 77 ITDs, and 76 fusions have mean read coverage values greater than 5 reads, Barnacle's default read-support threshold (see Stage 3, filter 8).
Mean read coverage within the differentiated region was lower in males (34.65, standard deviation = 10.56), compared to females (38.45, standard deviation = 12.00), but this difference was consistent with the total number of reads obtained from each sex.
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In a second experiment, 130,345,608 Illumina reads (mean read length 101 nt, total coverage 4 ×) were obtained.
We observed that multiple rRNA coding regions were fragmented in the assemblies from the second-generation sequencers, whereas PacBio generated two exceptionally long contigs of 3,288,561 and 1,875,537 bps, each of which was from a single chromosome, with 73× coverage and mean read length 3,119 bp, allowing us to determine the absolute positions of all rRNA operons.
PacBio long-insert libraries were prepared for both strains and sequenced to ~135× coverage for Bal225 (mean read length after adapter removal of ~ 2 kb) and ~200× coverage for DH5α (mean read length after adapter removal of ~2.4 kb).
Mean mtDNA read coverage per site ranged from 60x to 260x for DNA isolated from heart and was 7x for DNA isolated from blood.
We found that the mean coverage and read coverage distributions for the two sets of SNPs is virtually identical.
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