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Quantitative data were calculated from the Ct-values for each reaction using the mean reaction efficiency for each primer pair.
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The relative ratio of gene expression was calculated as follows: (1) Relative ratio = (Etarget ) DCt TARGET (mean comparator − mean sample ) (Eref ) DCt REF (mean comparator − mean sample ), where Etarget is the reaction efficiency of the gene of interest, Eref is the reaction efficiency of the reference gene and DCt is the cycle difference between the comparator and the sample.
Ct values were adjusted for starting concentration and reaction efficiency using the formula: 40 - (Sample Mean Ct Target + (Median 28S - Sample Mean 28S) * (Slope Target/Slope 28S)).
As the common means of DNA modification, click chemistry reaction was of advantages of high reaction efficiency, well-control, and facile operation [21].
However, the observation that thioester synthesis did not proceed to completion meant we could not rule out the possibility that competing NCL compromised reaction efficiency.
Fig. 3 Mean reaction time.
It was made of quartz to maximize the reaction efficiency.
Decreasing the reaction temperature and improving the reaction efficiency have been a great challenge to researchers.
The overall mean reaction time was 1.33±0.27 s (mean ± SD).
Reaction efficiency was 101.2%.
A reaction efficiency of 100% was assumed.
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