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Specifically, the baseline probe intensities were sampled from a distribution consisting of the mean raw probe intensities for each of the 201 800 probes used in this study.
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And we did not mean raw fish.
I mean raw, raw, raw!" The Afghan gave him a quizzical look, as if uncertain whether he was being chastised.
It simply means raw.
Technically, he was referring to their kids... but I figured raw chocolate-chip cookies meant raw chocolate is OK.
To give a couple of examples, the pm_mean value is the mean raw intensity for all probes on the array unusually bright or dim samples (high or low pm_mean values respectively) should be handled by normalization but this should be checked with probeset-level metrics as well.
The arrays were pre-processed by the GC-RMA algorithm using GeneSpring GX 11.0.2 (Agilent Technologies Inc .. Populus probes exhibiting mean raw hybridization intensities of at least 50 in any experimental group were flagged as "present", yielding a list of 24,871 probes, and the rest designated as "absent" and excluded from analysis.
The table gives the probe set ID, the gene symbol, the fold change relative to untreated mice, the direction of regulation, the mean raw signal values, the RMA-corrected signals, the Entrez gene ID, the description, and the chromosomal location of genes altered during the infection.
The raw probe set intensities were normalised by robust multi array average (RMA).
Raw probe summary data was exported into Microsoft Excel and transformed on the log base 2 scale.
Consequently, in almost all comparisons the median probe values differ by orders of magnitude and derive from raw probe values with no overlap.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com