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When ANCA was included, the difference in mean quartile sum lost significance.
In the final analysis, student t-test was performed to compare the mean quartile sum between cases and controls.
The mean quartile sum of the antibody responses was elevated in AS patients when ANCA was excluded (10.526 vs 9.519, p = 0.03).
The elevated mean quartile sum seen in AS patients was higher than controls when ANCA was excluded (10.526 vs 9.519, P = 0.03).
The following analyses were performed: comparison of positivity based on the established values in IBD, median values, the number of subjects in each serology in the 4th quartile of a normal distribution, and the mean quartile sum of all the antibodies.
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● Average weighted quartile sum indices were constructed based on the mean weights from 1000 bootstrap samples with positive and significant slope estimates and from 1000 bootstrap samples with negative and significant slope estimates.
By adding individual quartile scores for each glycan antigen a semi-quantitative quartile sum score (QSS, range 6 24) representing the cumulative quantitative immune response towards all six antigens for each patient was obtained.
The overall level of response to the antibodies was measured by summing the individual quartiles to yield a quartile sum.
Finally, we compared the quartile sum of all serologies in AS versus controls.
Interpretation of the weights from weighted quartile sum regression was instructed through simulation studies conducted by Carrico [ 11] (detailed below).
Instead, we use a nonlinear weighted quartile sum (WQS) approach [ 11] nested within a parametric proportional hazards model.
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