Your English writing platform
Discover LudwigSuggestions(1)
Exact(2)
The mean quantity values of the miRNA expression were normalized by U6 snRNA.
LOG transformation of PCR data of any kind is a necessity – it is the nature of PCR to show higher variability with lower mean quantity values due to the ever increasing Monte-Carlo effect with decreasing presence of target template in nucleic acid samples (2, 37).
Similar(58)
The mean quantity value for Spry2 or BDNF mRNA for each individual was normalized to the geometric mean of the three housekeeping genes to obtain the relative amount of each transcript in each individual.
Reactions were quantified by the delta delta cycle threshold (Ct) method using SDS2.2 software (Applied Biosystems, Foster City, CA) generating a mean quantity value (Qty mean) for each sample from the triplicates of that sample for each gene.
The duration of the effect was defined as the time-interval between the effect onset and the moment at which the mean PS quantity value returned to the baseline value.
The values obtained (mean quantity) for each target gene were divided by the respective values of the internal reference gene (ACTB).
To determine the relative levels of methylated promoter DNA in each sample, the values obtained by QMSP analysis (mean quantity) for each target gene were divided by the respective values of the internal reference gene (ACTB).
To determine the relative levels of methylated promoter DNA in each sample, the values obtained by QMSP analysis (mean quantity) for the target gene were divided by the respective values of the internal reference gene (ACTB).
The obtained quantity values were standardised to mean one by division with the median quantity value of the 15 examined probes (the median value was considered to be most likely to represent a normal copy number).
All quantity values were divided by the mean of the wild-type samples.
For each gene, standardized quantity values were calculated by dividing the mean of the quantity values for the three technical replicates for each sample by the mean of the replicate values of the RCP1 exogenous control for that sample.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com