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(b) Mean quality score of all sequences (Phred score) relative to a chosen threshold of 30, indicating a 99.9% chance that a base is called correctly.
The mean quality score of the reviewed articles was 50%.
All item scores were averaged to obtain a global mean quality score for each classroom.
Domain 5 ("Applicability") had a mean quality score of 46.3% with moderate variability (SD = 9.3%).
However, there is no statistical difference in the mean quality score between them (p = 0.3).
Domain 4 ("Clarity of Presentation") had the second-highest mean quality score (80.6%), with 9.3% SD.
The mean quality score of the Japanese studies was 6.7 and the proportion of high-quality studies (with a quality score higher than 8) was 32.6%.
Quality filtering of the joined sequences was performed and sequences that did not fulfill the following criteria were discarded: sequence length < 200 bp, no ambiguous bases, and mean quality score ≥ 20.
Our study showed that the mean quality score was 6.7 (e.g., a full score is 14), and 33% of Japanese PET studies were of high quality, as indicated by the quality score of more than 8.
The mean overall quality score for the total sample was 44 which is comparable to mean quality score of medical PILs of 35.2 reported by Rees et al. [14].
To correct these errors, for each possible base at each position in the locus, the mean quality score was calculated across all uniques.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com