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The mean protein staining intensity in breast carcinomas was determined to have an IRS of 5.9 (3.3) and the median to have an IRS of 6 (range 0 to 12).
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Coomassie Brilliant Blue R-250 was used for protein staining.
Ponceau S staining was performed for total protein staining.
& Nolan, G.P. Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events.
For each image, the total number of cells and the total Caveolin-1 signal were measured, and the estimated mean intensity of Caveolin-1 protein staining per cell was calculated by dividing the total Caveolin-1 signal, after subtraction of background, by the total number of cells.
Eat lean, mean protein.
Samples were then resolved on SDS-PAGE gels and proteins stained using colloidal Coomassie.
Total protein stain of PVDF membrane shows equal protein loading across groups.
The Stain Detector Light uses the same UV technology that the detectives use to find "protein stains", blood, bile, or, uh, what-have-you.
b Sample area with protein stain ×100.
After electrophoresis, the gels were stained using GelCodeTM blue safe protein stain purchased from thermo scientific.
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