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These results suggest that the mean protein length in complexes reflects to an observable degree the length distribution of proteins encoded in the proteome.
Like for the random human complexes, the mean protein length distribution for the real human complexes was also skewed towards smaller protein lengths.
Significant enrichment is not, per se, proof of function and could be for a trivial reason, such as strong amino acid bias or 4-fold increased mean protein length in transcriptional proteins.
Structural features of U{1 1 1 1} genes showed a mean protein length and exon number similar to features in U{1 1} genes as well as the same tendency towards a low TATA-box and a high TELO-box presence in promoters.
Other mean protein length values are presented in Table 3. Biological function has been proposed as a potential factor altering certain molecular evolutionary processes [ 10]; therefore we examined the gene profiles for each of the contrasting tissues for each species under study.
The predictions for the cucumber genome are a gene density of 10 per 100 kb, mean protein length of 349 amino acids, average number of exons per gene, exon length and intron length of 4.8, 238 bp and 483 bp, respectively, and tRNA gene density of 2.9 per Mb.
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Mean protein lengths (± Standard Error) for this category are as follows.
If mean protein lengths in complexes reflect protein length distributions, we expect hetero-oligomeric mitochondria human complexes with mean protein lengths over 1000 amino acids to be extremely rare.
The scarcity of large proteins in the mitochondria, in turn, will likely limit mean protein lengths of its complexes.
Although many (300/1962 = 15%) human complexes in our data have mean protein lengths over 1000 amino acids, none of the 28 mitochondrial human complexes we annotated so far exceed this limit.
In particular, when complex complexity increases past 20 different subunits, complexes with mean protein lengths below 250 amino acids are rarely generated randomly (Random Model 1: P < 10-6) but are present in annotated mammalian and yeast complexes.
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