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The position effects are significant, altering the mean protein expression level by up to 15 times and expression noise by up to 20 times.
In the present study, we monitored only mean protein expression changes between T1 and T2 of at least 30%.
was calibrated with recombinant firefly luciferase (Sigma) to give the mean protein expression of each PLCζ-luciferase construct [ 15].
Indeed, Figure 5C shows that a reduction of the mean protein expression to 50% of its constitutive level is sufficient to reduce the noise by approximately 40%.
The null hypothesis (Ho) was based on the assumption that mean protein expression levels of cancer and noncancer sets were the same.
As in Bar-Even et al. (2006), we find that protein expression variance scales roughly linearly with mean protein expression (Fig. 2).
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Results are expressed against the mean S100 protein expression in normal tissue samples; *p<0.05, **p<0.01, ***p<0.001.
Comparing the mean BOP1 protein expression between samples with 8q retention and 8q gain revealed a slight, although not significant, increase in the samples with gain (1.38 vs 1.16 relative protein expression).
E2 treatment decreased by 28% (P < 0.05) the mean AR protein expression level compared with control, whereas AR protein level was significantly increased following the combined E2+DHT treatment compared with the value noted following E2 treatment alone (P < 0.01).
The entry vectors do not provide a means for protein expression or functional assays; they are a place-holder for the ORF.
By WB, we observed that parkin mutant fibroblasts possessed a significantly higher mean of RTP801 protein expression compared with controls.
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mean protein level
mean protein intensity
mean protein length
mean protein excretion
mean protein binding
mean protein staining
mean protein band
mean protein sequence
mean protein value
mean protein content
mean protein abundance
mean protein similarity
mean protein amount
mean protein concentration
mean protein intake
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