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It is remarkable that the shift in mean probe intensity for the selected probes in fig 3 is so specific to the platform-primer combination studied.
This can for instance be seen in fig 3A where a much smaller shift in mean probe intensity is observed for those populations of probes that carry motifs from related, nearly identical T7 primers.
Mean probe intensity was determined for genes represented by more than one positive probe.
Log-ratio thresholds for amplification and deletion were set at 1.5 standard deviations from the mean probe intensity.
We used limma [ 34] to perform within-array normalization (using the "loess" method) and quantile normalization of mean probe intensity values (using the "Aquantile" method).
Between-array normalization was achieved using quantile normalization of mean probe intensity values, which is implemented by limma as the "Aquantile" method.
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However, motifs that are exclusively part of abundantly expressed genes will also have high mean probe intensities and ranks.
Interestingly, shifts in mean probe intensities for data obtained from the same Operon V 3.0 probeset vary depending on the T7 primer used.
We ranked the mean probe
Gender : for females and males separately, the mean probe intensities across SNPs on chromosome X.
Comprehensive RNA degradation plots of individual mean probe intensities indicated comparable transcript integrity across all samples and culture periods (Additional file 1a).
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mean probe start-to-start
mean probe delay
mean drought intensity
mean probe fluorescence
mean probe space
mean probe spacing
mean fluorescent intensity
mean probe signal
mean staining intensity
mean light intensity
mean probe value
mean smoking intensity
mean probe summarization
mean background intensity
mean pain intensity
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