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Mean population doubling 0 was arbitrarily defined as the time point when the infected cells reached 80 90% confluency in selective growth media.
In this context, the average doubling times of HT-29 and DU-145 cultures during their exponential growth phase were calculated to be 13 16 h or 22 h, respectively [ 8- 10], whereas the mean population doubling times of renal or pancreatic carcinoma cell lines ranged between 24 104 h or 16 40 h, respectively [ 11- 14].
Three goats received a membrane with goat chondroprogenitors (mean population doubling, 21.4) whilst another three goats received a membrane seeded with full-depth goat chondrocytes (mean population doubling, 3.5).
Cells generated from the second dish were counted as passage 1, and mean population doubling times (mpds) were determined from this point.
The first plate to reach confluence under selection was arbitrarily defined as mean population doubling (mpd) 0. Retroviral infections were used to establish polyclonal BJ cell lines that stably expressed pBABEpuro, pBABEpuro-FLAG-hTERT WT, or pBABEpuro-FLAG-hTERT Q169A.
The mean population doubling time (PDT) for 91.1.F1 was around 90 h.
Similar(51)
In TERT-positive tumour cells between population doubling levels of 11 and 169, the mean telomere length continuously increased from 12 kbp to 24 kbp (Fig. 1A).
The population doubling time varied between 0.49 and 1.22 per day with a mean of 0.77.
Maximum EcadAb ES cell expansion was achieved following 14 d in culture, with a mean fold-increase in cell number of 25.3±0.9 over a 72 h period with resultant population doubling times ranging between 15.6 h and 27.1 h.
Cells underwent a population doubling of 2.1-fold, with a population doubling time of 35 h.
Population doubling times varied from 48 72 h.
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