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Mean pneumococcal density among pneumococcal nasopharyngeal carriers was calculated before and after vaccination.
Mean pneumococcal density was compared in CSSs conducted before and after vaccination.
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Mean pneumococcal colonisation density was not significantly different between patients with and those without antimicrobial activity in urine (3.36 (95% CI 2.82 to 3.89) copies/mL vs 3.95 (95% CI 3.30 to 4.60); p=0.16).
Pneumococcal density was defined using a semiquantitative classification (range, 1 4) among colonized individuals.
In addition, there was no consistent difference in pneumococcal density among the postvaccination surveys.
The pneumococcal density correlation between culture and qPCR was determined for 147 samples.
Pneumococcal density fluctuated within a carriage episode, and occasionally fell below the detection limit of both methods.
Pneumococcal density in nasal washes collected at six time-points post-inoculation was determined by culture and quantitative PCR (qPCR).
The estimation of pneumococcal density by culture and qPCR was highly correlated (rs = 0.73, p <0.0001), although qPCR had a lower detection limit.
We detected a higher pneumococcal carriage rate with qPCR than with culture but pneumococcal densities measured with the two methods were positively correlated.
The parallel decreases in culture positivity rates and pneumococcal densities measured by qPCR suggest that qPCR is more suitable for detecting low levels of carriage.
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