The mean pixel gray-level value in each sector was then calculated as an estimate of the mean amount of bone mineral in this sector.
Using the MRIcro software program, five regions of interest were drawn on each image, and the mean pixel signal intensity level was recorded.
For assessment of collagen I protein levels, mean pixel intensity of collagen I IF was measured over the tumor periphery FOVs.
The mean confidence interval at the pixel level for the LIDAR model was 246 Mg/ha and 84% of the plots had a confidence interval that overlapped the confidence intervals of the corresponding pixels.
However, MSB (E, E), MSB (E, F) and MSB (E, G) were at 104 − 106 pixel level, which means that if one observation for GCPs' world coordinates was contaminated by one outlier, the bias on world coordinates at least needed to range from 0.052 m to 5.20 m in order to be separated as the size of one pixel in this simulation was 5.2 um.
The mean pixel density was measured using the 256-level greyscale of the 'Histogram' function in Adobe Photoshop 7.0 (Wada et al., 2004; Mouritsen et al., 2005).
Protein levels were determined by averaging mean pixel intensities measured with Un-Scan-It software (Silk Scientific, Orem, Utah) from three independent western blots.
We evaluated the root mean square errors and significance of biases at the pixel level in order to determine the optimal parameters.
