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The mean PIC value of 0.60 over 25 loci shows that the majority of the microsatellite loci are highly informative.
And, allele number was ranged from 4.81 to 10.5 and mean PIC value from 0.46 to 0.62 for above-mentioned studies.
By contrast, mean PIC value calculated based only on markers scores obtained in landraces, the most diverse germplasm subgroup of S. cereale ssp.
The mean PIC value of all 18 cgSSR primers in the present study was 0.278 which is higher than the previous report describing salt responsive miRNA-SSR markers in rice [ 31].
This lower than expected mean PIC value can result from certain limitations of DArT markers in analyses of heterozygous and heterogeneous samples [ 19], that will be discussed in more detail later, and from germplasm choice [ 45].
PIC values ranged from 0.0 – 0.78 with a mean PIC value of 0.47 ± 0.17.
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Mean PIC values were compared with Wilcoxon test (α = 5%).
In WOSR, the mean PIC values were not very different between the three seed quality types.
The inclusion of DArT clones from various genomes meant the average PIC value of the data set (0.31) was lower than in comparable studies.
However, in our study, the mean difference of PIC value between di-nucleotide and tri-nucleotide containing cgSSRs was not quite statistically significant (p value 0.0627).
Mean PIC, MAF, and GD values were 0.35, 0.25, and 0.36, respectively (Additional file 4: Figure S3).
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