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Mean peak transient I V relationships for WT and V287R are illustrated in Figure 1.A.
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In four independent experiments, only 26% of recorded cells showed a transient rise in [Ca2+]i, along with a significant reduction in mean peak amplitude (191 ± 14 nM versus 444 ± 18 nM; n = 23, P < 0.001) and a delayed latency (1147 ± 31 s versus 537 ± 35 s; n = 23, P < 0.001) (Fig. 8A).
Analysis of average Ca2+ transient revealed no significant differences in peak transient amplitude between isoforms.
In addition, transient increases in supine heart rate were apparent 3 8 h postdose, with the mean peak increases from baselines ranging from 10 to 17 beats min−1 among the groups.
Mean peak latency was 2.2 ± 0.2ms.
Their names indicate their polarity and mean peak latency.
For simulations with 100 µM OGB1 but without calbindin-D28k, the mean peak [Ca2+]i (i.e. the average amplitude of all peaks, independent of when they occur) was 1.07±0.7 µM but the peak of the averaged calcium transient was only 0.17 µM (Figure S3B).
Peak transient sodium currents were measured after subtraction of scaled passive current responses to the appropriate voltage steps.
Mean peak currents are shown.
The mean peak pressure was 20 ± 5 cmH2O.
(F ) Amplitude of late NaV current as a % of peak (transient) NaV current.
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