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The mean peak heights were obtained after triplicate sample aspiration.
The mean peak heights were compared with those obtained with 1.0×10−3 mol L−1 pure standard NLX.
region within the same probe spot also appeared similar (Figure 5C), though with a modest increase in mean peak heights and diameters (Table 2).
The mean peak heights between cells not treated and those pre-treated with thapsigargin, for both NG and HG culture conditions, were each reduced by approx. 60%.
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Acquisition captures all peaks, overlays them and finds the mean peak height to calculate particle charge.
A number of surface roughness parameters, viz., the center line average (CLA, Ra) and root mean square (RMS, Rq) roughness, maximum mean peak-to-valley height (Rtm), maximum mean peak height (Rpm), maximum mean depth (Rvm), and average asperity slope (Δa) were calculated.
The charge measurement is performed by calculating the mean peak height in volts of the image charge waveform induced by the trapped particle (Fig. 9) in a 64 ms data acquisition window.
From the profile ordinate data read at 1 μm intervals, a number of surface roughness parameters – centre line average, root-mean-square roughness, maximum height, maximum mean peak height, maximum mean depth, and absolute average asperity slope were calculated using a computer program.
Using an optimal range of DNA (0.5 1.0 ng), validation studies revealed well-balanced electropherograms (inter-locus balance range: 0.500 1.296), relatively balanced heterozygous peaks (mean peak height ratio of 0.83 across all loci) with minimal artifacts and stutter ratio (mean stutter of 0.021 across all loci).
SNR was calculated by a mean peak height of PSF (11.15 photons) divided by the standard deviation of noise (in photon).
The replicate analyses of the pool also provided an estimate of the coefficient of variation associated with each peak, that is, the standard deviation of the peak height across pool replicates normalized to mean peak height.
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