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Peak height values were normalized across samples by dividing by the mean peak height across all metabolites.
From the profile ordinate data read at 1 μm intervals, a number of surface roughness parameters – centre line average, root-mean-square roughness, maximum height, maximum mean peak height, maximum mean depth, and absolute average asperity slope were calculated using a computer program.
Using an optimal range of DNA (0.5 1.0 ng), validation studies revealed well-balanced electropherograms (inter-locus balance range: 0.500 1.296), relatively balanced heterozygous peaks (mean peak height ratio of 0.83 across all loci) with minimal artifacts and stutter ratio (mean stutter of 0.021 across all loci).
A number of surface roughness parameters, viz., the center line average (CLA, Ra) and root mean square (RMS, Rq) roughness, maximum mean peak-to-valley height (Rtm), maximum mean peak height (Rpm), maximum mean depth (Rvm), and average asperity slope (Δa) were calculated.
Acquisition captures all peaks, overlays them and finds the mean peak height to calculate particle charge.
The charge measurement is performed by calculating the mean peak height in volts of the image charge waveform induced by the trapped particle (Fig. 9) in a 64 ms data acquisition window.
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The mean peak heights were obtained after triplicate sample aspiration.
The mean peak heights were compared with those obtained with 1.0×10−3 mol L−1 pure standard NLX.
region within the same probe spot also appeared similar (Figure 5C), though with a modest increase in mean peak heights and diameters (Table 2).
The mean peak heights between cells not treated and those pre-treated with thapsigargin, for both NG and HG culture conditions, were each reduced by approx. 60%.
For example, each component in a doublet had a scaling factor of 2, whereas those in a triplet were 4, 2 and 4. The mean peak-height value calculated from the fine structure components for each cross-peak was taken as the peak-height value for that cross-peak.
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