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Velocity (curvilinear and mean path velocities) and linearity of cryopreserved spermatozoa are generally reduced [ 10].
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Tracks in 10 fields were typically recorded for each mouse, along with mean values for average path velocity (VAP, µm/sec), straight line velocity (VSL, µm/sec), curvilinear velocity (VCL, µm/sec), amplitude of lateral head displacement (ALH, µm), beat cross frequency (BCF, Hz), straightness (STR), and linearity (LIN).
MOT = motility ; HYP = hyperactivated sperm ; VCL = curvilinear velocity (μm/s); VSL = straight-line velocity (μm/s); VAP = average path velocity (μm/s); ALH = mean amplitude of head lateral displacement (μm).
VSL: straight line velocity, VAP: average path velocity: VCL: curvilinear velocity.
The variables measured included: average path velocity (VAP), straight line velocity (VSL) and curvilinear velocity (VCL) [ 87].
VCL, Curvilinear velocity; VSL, Straight-line velocity; VAP, Average path velocity; ALH, Amplitude of lateral head displacement; LIN, Linearity; STR, Straightness; BCF, Beat cross frequency.
VCL = curvilinear velocity, VSL = straight-line velocity, VAP = average path velocity, LIN = linearity, STR = straightness, WOB = wobble, ALH = amplitude of lateral head displacement, BCF = beat cross frequency.
Progressive cells were defined as average path velocity >25 µm/s and straightness >80%.
Motility data is further characterized as follows: mean curvilinear velocity (VCL), path velocity (VAP), mean straight-line velocity (VSL), straightness (STR = VSL/VAP), linearity (LIN = VSL/VCL), percentages of total motility (MOT), progressive motility (PMOT), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF).
Mean path loss.
Arterial pulsatility index (PI) was defined as (maximum velocity – mean velocity)/maximum velocity.
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