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Under the model of rapid population expansion, gene (h) and nucleotide diversities and the mean pairwise sequence differences between the haplotypes (d) are expected to be low [26], [27].
Mean pairwise sequence divergence within Rattus spp. rat HEV genotype 3 sequences was 0.51%.
2 families had no sampled alignments with a mean pairwise sequence identity larger than 60%.
Mean pairwise sequence divergence within each cluster was calculated as an estimate of Θπ.
Numbers of Sequence (N), Segregating Sites (S), Mean Pairwise Sequence Differences (MPSD) and Nucleotide Diversity.
Mean pairwise sequence identities within each family are between 0.2 and 0.3 (Additional file 9), so the families are roughly comparable in sequence diversity.
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The average pairwise sequence identities range from 27.2% to 66.2% with a mean of 42.5% and an SD of 8.5%.
The average pairwise sequence identities within specificity groups vary from 25.5% to 88.9% with a mean of 55.2% and an SD of 14.2%.
The first metric is used to remove divergent protein sequences, defined as sequences with mean identity (mean of pairwise sequence identity values when comparing a sequence against all others within group) smaller than user-defined cutoff.
Sequence identity levels were calculated as the arithmetic mean of all pairwise sequence comparisons in the untrimmed multiple sequence alignment of a given Hsp70 subfamily.
In contrast to the high copy number dynamics, sequence evolution of SSA genes was very slow, as illustrated by the short branches of the protein tree (figs. 1 and 2 A) and the high level of mean pairwise amino acid sequence identity (∼82%, table 1).
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