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With this software the mean optical background level for each array was subtracted from the signal intensity for each probe.
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Mean optical density (with background correction) throughout the Z-stack of FGF2 expression in the DG revealed no effect of stress or CORT injection on DG FGF2 expression.
Given the ubiquitous and closely-packed FGF2 immunoreactivity in the DG, we had to measure mean optical density (with background correction) throughout the Z-stack of FGF2 expression in the DG (rather than in individual cells).
Data were presented as the mean optical density (OD) corrected for background (wells without coated antigen).
Background subtraction was performed using the mean optical density value from 4 arbitrarily selected, vessel-free, areas (60×60 pixels).
Mean optical density of FGF2- areas of tissue were subtracted from the DG intensity value to correct for background.
An ANOVA model was used to compare mean optical density among different exposure durations.
Appropriate background corrections should consider the optical background and partly also non-specific hybridization.
Background intensity contributions (optical background and "chemical" background due to non-specific hybridization) should be considered especially for probes forming at least one mismatched pairing.
Graphed data are presented as the mean optical density (group means mGluR5/actin immunolevels) ± the standard error of the mean (SEM).
and the mean optical density (M.O.D).
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