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a Mean of treated to control ratios measured by microarray in 4 independent experiments.
Dental data were reported as mean of treated teeth and the different types of treatment provided.
For each probe set, the fold change in expression was calculated by subtracting the mean of control samples from the mean of treated samples.
Plates were then read at OD 540 nm, using a microplate reader (Synergy HT Micro Plate Reader, BIO-TEK INSTRUMENTS, USA) and the results expressed as a percentage cell viability (mean of control group – mean of treated group/control group × 100%%).
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The differences between the means of treated and untreated groups were considered significant at p < 0.05.
Comparisons of the differences between the means of treated and untreated samples of qRT-PCR data were performed using Student's t-tests (p-value ≤ 0.05 except for the data marked with ns).
Difference between Means of treated and control groups was considered significant at P < 0.05.
The differences between the means of treated and control groups were tested for significance using Fisher's least significant differences at P ≤ 0.05 (Fisher PLSD).
The ANOVA on log-transformed counts showed significant effects of strategic treatments on immature Ostertagia (p < 0.05), Trichostrongylus (p < 0.0001) and total counts (back-transformed least square (LS) means of treated: 4358 and untreated: 8102; p < 0.05).
For each variable, we also identify whether the difference in the mean values of treated and non-treated individuals is significant.
Mean values of treated groups were compared with mean values of a control group and analyzed using statistical methods.
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