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This was measured for a minimum of 70 Sertoli cell nuclei per testis, and mean nuclear volume was then determined.
Red dagger indicates that mean nuclear volume of R11ΔS15 is significantly smaller than its isogenic WT strain (ΔS15; p<0.001).
The mean nuclear volume of Leydig cells and gonocytes was measured using the stereology program, which calculates volume based on the assumption that all objects are spherical.
The aim of this study was to obtain morphometric data that would enable objective and reproducible grading of prostatic cancers by stereological estimation of mean nuclear volume (MNV).
Again, there was no significant difference in mean nuclear volume per gonocyte between T (294.7 ± 32.7 × 10−6 μm) and C (310.1 ± 29.1 × 10−6 μm) testes.
The mean nuclear volume per Leydig cell for T testes (122 ± 14.7 μm), however, was similar to that for controls (112 ± 14.5 μm).
Similar(53)
Differences between mean nuclear volumes were assessed by the Welch t-test to calculate 95% confidence intervals and p-values.
3-D confocal imaging and measurement of the nuclear volume confirmed that LmnaDhe/+ cells had a larger average nuclear volume than Lmna+/+counterparts (p≤0.001, Student's t-test).
Although there was a trend with p values of 0.07, the mean global neocortical perikaryon volume was not significantly larger in men compared with women and the mean neuronal nuclear volume was not significantly different in the two sexes.
In wild type hermaphrodites the X chromosome territories were compact with a mean percent nuclear volume of 10.31 ± 1.98%.
H3f3b KO MEFs exhibit a greater than 40% mean increase in nuclear volume, but a more compact morphology as measured by shape factor suggesting that loss of H3f3b may affect nuclear architecture/chromatin structure overall.
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