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Reflectance spectroscopy can probe changes in epithelial nuclei that are important in pre-cancer detection, such as mean nuclear diameter, nuclear size distribution and nuclear refractive index.
To quantify cell cycle changes in cell volume, we compared the mean nuclear diameter of cells with a single nucleus before mitosis (di) (Fig. 1, b c) and in cells with two nuclei after (df) mitosis (Fig 1g) (Table S1).
The mean nuclear diameter of 100 breast cancers was measured on tissue sections, to evaluate its importance for early prognosis.
For the mean nuclear diameter, these islets were then examined to identify five representative β-cell nuclei each, as previously described (13, 14).
Studies of the parathyroid gland have indicated a relationship between parathyroid hormone secretion and the mean nuclear diameter of parathyroid cells (21).
The cell numbers were corrected using Abercrombie's method [ 32] by taking into account the thickness of the section, actual profile count, and mean nuclear diameter.
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Data were converted to absolute volume per testis by multiplying by testis weight (equivalent to volume), then converted to cell number per testis after determination of mean cell nuclear diameter and volume (average of 70 100 nuclei) using the Stereologer software nucleator function.
That study found positive associations for mean nuclear area, nuclear diameter, nuclear perimeter, and the longest nuclear axis, but no associations for SD of the nuclear area and the shortest nuclear axis; shape factors were not evaluated.
From the knowledge of section thickness and mean neuronal nuclear diameter of labeled neurons in a certain brain region, we used the Abercrombie stereological correction [ 45] to estimate the number of [H]-thymidine+ neurons per mm in each brain region.
Since mean neuronal nuclear diameters were similar across most groups, we can assume that neuronal age was also similar and, hence, that the [H]-thymidine+ neurons were born within the 5 days of treatment.
Although there was no significant change in β-cell size with aging, the mean β-cell nuclear diameter increased with age (r = 0.6, P < 0.0001) (Fig. 4).
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