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The binning window to assess the mean normalized depth is 100 kb.
Pearson correlation of mean normalized depth between two replicates amplified by the same WGA kit.
Overall, the mean normalized depth distribution biases for DOP-PCR methods, MDA-2 and MALBAC were lower than those of MDA-1 and MDA-3.
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We then found that the mean normalized sequencing depth distribution of DOP-1 data was most similar with the theoretic one, whereas all other amplification kits had observed bias (Fig. 2b).
The mean normalized read-depth over each bp position was used to construct a plot of the average TSS profile.
Values are mean normalized coverage.
The NCR was normalized to control for each biological replicate before pooling, and plotted as mean normalized log (NCR) ± s.d.
Statistical analysis was done using repeated measures ANOVA on the mean normalized CT values (n = 3).
Data points represent mean normalized calcium content (μg Ca per μg DNA) ±standard deviation, n = 3 7.
The horizontal and the vertical axes represent normalized horizontal position and normalized depth, respectively.
(b) Mean normalized difference of CRLBs vs. burst length K ′.
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