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The limit of detection (mean negative control plus 3 × standard deviation) was determined for each protein assay.
The sample/positive-ratios (S/P-ratios) were calculated based on optical densities (OD) as follows: S / P = OD sample - OD mean negative control OD mean positive control - OD mean negative control Data were collected using accordingly structured and standardised data collection forms in compliance with the guidelines for 'Good Clinical Practise GCPCP) [ 15].
The expression of EGFRvIII was categorized using NS as absent (mean negative control counts, see " Methods") or present as a fraction of overall EGFR: <1 % (black), 1 10 % (orange) or ≥10%% (red).
Responses were measured as specific spot forming units (SFU), calculated as the difference between the mean treatment spot count and the mean negative control spot count.
A detectable response was defined as a mean treatment spot count that was more than 10SFU 105 cells−1 greater than twice the mean negative control.
Previous studies screening for tumor-specific antibodies have selected wells greater than the mean negative control optical density (OD) + three standard deviations as criteria for positive tumor-reactive antibodies [39].
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The results were expressed as S/P, calculated as (mean of sample OD450 - mean of negative control OD450)/ mean of positive control OD450 - mean of negative control OD450) × 100.
Cell viability for each sample was calculated using (3) Cell survival % = Mean of each group − mean of blank mean of negative control − mean of blank × 100.
The results are expressed as spot-forming units (SFUs) per million PBMCs calculated by subtracting the mean R10 negative control response from the mean peptide pool response.
The resulting ECL signal/log competitor concentration curves were analyzed by four-parameter logistic regression where the top and bottom asymptotes were constrained as the ECL value obtained from the un-competed sample mean and the negative control mean, respectively, and the resulting IC50 value was reported.
For each biomarker, the limit of detection (LOD) was defined as the mean of negative control plus 3 standard deviations of the 38 negative controls.
More suggestions(15)
mean negative curvature
mean effective control
mean negative Capability
mean negative free-energy
mean judicial control
mean negative score
mean negative result
mean negative angulation
mean glycaemic control
mean negative change
mean negative pressure
mean ∆Ct control
mean Abs control
mean corporate control
mean central control
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