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An analysis of migration velocity showed that LIII +ecdysone hemocytes migrated at velocities ranging from 0.14 0.81 µm/s, with a mean migration velocity of 0.39 (SE±0.02) µm/s.
While the primary keratinocytes and early HF1 cells were highly motile (typical mean migration velocity: 0.35 µm/min), late HF1 cells barely moved throughout the experiment.
Analysis of the CT26 non-target shRNA control cells showed a mean migration velocity of 32.1 μm/h and a directionality of 0.66.
The mean migration velocity < v> was calculated according to the expression: At least three independent experiments were performed for each protocol.
There was significant difference (P<0.001), determined by a Student's T-test, to LIII hemocyte mean migration velocity at 0.21 ±0.01) µm/s (Fig. 2C).
This was comparable to WPP hemocyte mean migration velocity at 0.43 (±0.02) µm/s, and also closely similar to previously reported WPP in vivo migration velocities (Moreira et al., 2013).
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The track parameters calculated from these morphometric parameters include persistence, effective velocity, average migration velocity, lamellar activity, and overall directionality.
The amount of the diffusive matter during deformation is calculated for an aggregate of spherical grains and is converted to the increment of mean boundary migration velocity.
The median slope across replicates then yields the median migration velocity for a condition.
The average cell migration velocity emerged as a crucial parameter for the model.
We tracked individual cells and measured their migration velocity and mean distance.
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