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The mean microvascular density was calculated for each histological group.
Mean microvascular density was 12.5±1.2 in the control group and 14.3±1.4 in the rhEPO group (P=0.35, Student's t-test).
As shown in Table 3-wrap>, mean intratumoural microvessel density was 11.9±3.7 (median 12), IMD was independent from nodule size (P-value=0.89) being significantly higher in sarcomas than in colon cancer (mean microvascular density: 16.6±1.7 vs 10.2±3.2, respectively; P<0.0001).
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Tumour sections were stained with a pan-endothelial antibody (vWF) and vascularity was quantitated, without knowledge of the clinical details, by three methods: highest microvascular density; average microvascular density; and average microvascular volume.
Microvascular density was calculated as the mean number of stained vessels in 10 high-power fields (× 400).
Quantification of stained vessels can be achieved by measuring highest microvascular density (h-MVD), the average microvessel density (a-MVD) or the microvascular volume (MVV).
Microvascular density.
LPS, E. coli lipopolysaccharide; ERP, early resuscitation protocol; MVD, microvascular density; PVD, perfused vascular density; MFI, microvascular flow index; HFI, heterogeneity flow index; PPV, proportion of perfused vessels.
Microvascular density (MVD) was determined as the number of vessels per mm2.
The analysis of the microvascular density was restricted to vessels with a diameter <20 μm.
Analysis of microvascular density parameters is time consuming and operator-dependent.1 This is the main limitation to use microvascular monitoring in clinical practice as a "point-of-care" tool.
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