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Fig. s3 Mean methylation versus age and DS status.
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The methylation ratio of each CpG site for each tissue was calculated as the percentage of methylation versus the methylated plus unmethylated sites.
Table 1 Mean methylation fractions for BDNF CpG units in bipolar disorder versus controls Amplicon CpG unit BP MMF (SD) Controls MMF SDD) Z Asymp.
No significant difference in AS2 methylation was found between PHPΔstx16 and PHPneg patients with mean methylation values of 10%95%95 % confidence interval (CI) 7 13 %) versus 8%% (95 % CI 5 11%%), respectively.
MMF, mean methylation; d, effect size; Z, Mann Whitney U test statistic.
GATA binding protein 2. GATA binding protein 3. Mean methylation fraction.
The mean methylation level of regenerants showed a 0.28% to 3.95% decrease in seedlings subcultured in vitro for nine generations.
We examined DNA methylation for each CpG promoter region using the average of all CpG units mean methylation fractions (MMFs) in a BDNF region that we studied, in this case, the amplicons that were assayed.
The remaining tissues demonstrated partial mean methylation levels; more specifically, saliva − 0.43, vaginal fluid − 0.22 and menstrual blood − 0.22.
However, to what extent the observed synergy is due to changes in DNA methylation versus changes in protein methylation, or both, remains to be determined.
For analysis of age-associated DNA methylation changes we have calculated mean methylation of samples from young and elderly donors.
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