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Thus statistically the proposed mean methylation thresholding method may be considered equivalent to cytology.
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Researchers just need to define the analysis parameters, such as the covariates to be used in the ANOVA/MANOVA, the FDR correction method, the significance threshold and the minimum difference between mean methylation values between the groups under investigation.
As the mean methylation has the best performance, in terms of ROC curve analysis, the next step was to identify a threshold and separate the histologically benign from histologically malignant cases.
MMF, mean methylation; d, effect size; Z, Mann Whitney U test statistic.
GATA binding protein 2. GATA binding protein 3. Mean methylation fraction.
The mean methylation level of regenerants showed a 0.28% to 3.95% decrease in seedlings subcultured in vitro for nine generations.
We examined DNA methylation for each CpG promoter region using the average of all CpG units mean methylation fractions (MMFs) in a BDNF region that we studied, in this case, the amplicons that were assayed.
The remaining tissues demonstrated partial mean methylation levels; more specifically, saliva − 0.43, vaginal fluid − 0.22 and menstrual blood − 0.22.
The mean methylation level of the R0 plants was 12.78%, whereas that of the R9 plants decreased by 0.28%3.95%% compared with that of the R0 plants (Table 5).
For analysis of age-associated DNA methylation changes we have calculated mean methylation of samples from young and elderly donors.
Mean methylation level of duplicates was used.
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