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The methylation status of the SLC6A4 promoter in whole blood from pregnant women and newborn infants observed in this study were within the same range previously reported by others for the mean methylation status of the SLC6A4 promoter in lymphoblast cell lines [18].
Finally, the mean methylation status of the fragments was determined by amplifying the fragments by quantitative real-time PCR.
In case two CpG loci were present for one gene (MGMT and RB1), the mean methylation status was calculated for further analysis.
CD11a mRNA levels were negatively correlated with the mean methylation status of the promoter region in the 5-azaC-treated cells.
The mRNA levels of CD11a were negatively correlated with the mean methylation status of the 23 CG pairs in the promoter region in SSc patients (r = -0.577, P =0.012, Figure 1G).
As the Infinium 450k provides an estimate of the mean methylation status of the DNA hybridized to the array, it is difficult to say if only the extra copy of HSA21 is aberrantly methylated or if DNA methylation changes affect all the 3 copies of HSA21.
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M + and M- means methylation positive and methylation negative, respectively.
In this study, we investigated functional brain changes to novel visual stimuli of suicidal means, DNA methylation status, and the relationship between the two markers.
Finally, the mean methylation or hydroxymethylation status of the fragments was determined by amplifying the fragments by quantitative real-time PCR.
We employed melting profile analysis as a means to assay for methylation status.
In CLL, correlation between miR-124-1 miR-124-1 miR-124-1with continuous (methylationean diagnostatusaemoglobin, lymphocyte and platelet counts) and categorical variables (gender and Rai staging) with studied by Student t-test and continuous test (or Fisher Exact test) respectively.
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mean meeting status
mean methylation beta
mean hydration status
mean cognitive status
mean methylation score
mean methylation quantity
mean methylation thresholding
mean landmark status
mean methylation change
mean health status
mean methylation versus
mean performance status
mean baseline status
mean oxygenation status
mean methylation level
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