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The mean methylation quantity of the informative CpG sites per each gene was used to figure out the methylation proportion of the candidate genes (Dataset S1).
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On pyrosequencing, only four samples (4/78) demonstrated mean methylation quantities greater than 15%.
To generate delta methylation histograms for WT vs. WTdCas9 cat3a cells, we compared mean methylation over 1 kb tiles.
Together, our single cell analysis shows that post-replication methylation delay reduces mean methylation of individual cells in S-phase.
Globally, DKO cells are hypomethylated in comparison to WT (mean methylation shown on top left).
(b) Mean methylation levels for WT and DKO nascent and bulk DNA is shown.
(e) For CpGs located within specific genomic features, the ratio (nascent to bulk methylation) is shown on the x-axis and bulk mean methylation on the y-axis.
(l) For CpAs located within specific genomic features, the ratio (nascent to bulk methylation) is shown on the x-axis with bulk mean methylation on the y-axis for features as defined in e. Supplementary Figure 2 Effect of lag on single-cell methylation levels and nascent strand methylation dynamics.
Most cells in G1 (blue) or G2-M (green) show higher mean methylation than cells in S-phase (grey).
MMF, mean methylation; d, effect size; Z, Mann Whitney U test statistic.
Mean methylation levels of multiple CpG sites in the promoter regions were used for analysis; 15% or more was defined as methylation positive.
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