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Differences were small; 69 % of the significant sites differed in their mean methylation beta value by ≤0.2.
a Individual and mean methylation beta values of EN1 CpG island and the adjacent CpGs (chr2: 119,604,000–119,606,000).
Of samples in Batches 1 and 2, 6 were excluded based on call rates, and one failed bisulfite conversion; in Batch 3, 10 samples were removed following quality control (9 samples failed the bisulfite conversion, one sample with low mean methylation beta value across probes).
Despite statistically significant differences, we found that the majority of these CpG sites did not differ greatly in their overall level of methylation; 69 % of the statistically significant sites (124,392) showed a difference in their mean methylation beta value (delta beta; dβ) of less than 0.2.
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First, a false discovery rate (FDR) below 0.01 (Mann-Whitney U test) and a mean DNA methylation (Beta) values between tumors and controls showing a difference of at least 0.3.
MCaM, MCoM represent the mean of case methylation (Beta) and mean of control methylation (Beta).
Initially, we have selected CpG sites with differences in average beta value greater than 0.15 (more than 15% difference in mean methylation level) as this method is relatively resistant to potential confounding BeadChip effects.
Each beta mixture component is referred to as a methylation state and represented by its mean methylation level.
MMF, mean methylation; d, effect size; Z, Mann Whitney U test statistic.
GATA binding protein 2. GATA binding protein 3. Mean methylation fraction.
The mean methylation level of regenerants showed a 0.28% to 3.95% decrease in seedlings subcultured in vitro for nine generations.
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