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First, when direct recordings were made from teased peripheral nerve fibers in a skin-nerve preparation after the application of rapamycin or control solution, rapamycin increased the mean mechanical threshold for A-fibers and C-fibers by 58% and 54%, respectively, indicating that rapamycin had an effect on subpopulations of both C- and A-nociceptive fibers (Jimenez-Diaz et al., 2008).
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However, there exists no experimental method for determining the mean mechanical pain threshold of intradental nociceptors.
The mean of mechanical thresholds or thermal latency for each group of animals was compared at each individual time point.
The test was repeated three times for each rat at approximately 5-minute intervals, and the mean values of the three repetitions were counted as the mechanical threshold.
Mechanical threshold of muscle input ranged from 8.8 to 14.7 mN (mean temporal muscle 12.7 mN, neck muscles 13.6 mN).
The mechanical threshold determined with graded von Frey filaments ranged from 0.49 to 11.8 mN (mean 5.0 mN).
Mechanical threshold (MT) testing is widely used to measure nociceptive thresholds.
After the waiting period, participants underwent the three quantitative sensory tests for pain: heat pain threshold and tolerance, cold pain threshold and tolerance, and a second mechanical threshold.
Mechanical threshold was tested using von Frey filaments (Stoelting, Kiel, WI, USA) in a blinded manner.
Then, the change in the mechanical threshold (CMT, %) was calculated.
The flexion-elicited mechanical threshold was expressed in grams (g).
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