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Linkage analysis confirmed linkage of the above ten markers onto the same linkage group (SA-LG2), with a total length of 64 cM and mean marker interval of 7.09 cM.
The map covered 2,081.5 cM with a mean marker interval of 12.3 cM.
At MMU1 an additional 13 markers were genotyped, giving a total of 21 markers covering 51.8 cM, with mean marker interval of 2.59 cM; while at MMU5 an additional 14 markers were genotyped, giving a total of 20 markers covering 53.0 cM, with mean marker interval of 2.79 cM (see Figure 2 for details).
The F2-based map consisted of 13 LG spanning 1,012 cM, with a mean marker interval of 3.1 cM, where the largest gap resided in Chromosome IV associated with LG 4A and LG 4B. a LG = linkage group.
Of the 60 SNP markers developed for fine mapping in the region containing the tet-o locus, 52 could be successfully mapped with a mean marker interval of 0.5 cM.
For each map, progeny size (n), map length (cM; total for all 11 linkage groups), mean marker interval (MMI; average for all 11 linkage groups) and total number of mapped markers (using only those linkage groups included in composite map construction; see Methods) are given.
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The genetic distances of seven linkage groups spanned 66.12 cM (LG7) ~ 193.65 cM (LG3), with mean marker intervals ranged from 0.40 cM to 0.58 cM.
The average marker interval was 9.69 cM calculated as the arithmetic mean of the map distances between adjacent markers (Table 1).
The average marker interval was 5.8 cM.
Average marker interval was 10.6 cM.
The mean and median marker interval for the genome-wide SNPs was 107 Kb and 70 Kb, respectively.
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