Fig. 4 showed that as mean molecular marker expression levels increase, radiological response becomes poorer.
Two groups will always be compared using several cut-off points in the number of cells or in the level of molecular marker expression: mean, first quartile, second quartile, third quartile or the presence or absence of stained molecular markers.
Measurement of cell surface marker expression (mean fluorescence intensity, MFI) and viability was performed by four-colour flow cytometry (FACS Calibur, BD Biosciences, San Jose, CA, USA) using specific antibodies for CD11b, CD14, CD36, CD206, CD40, HLA-DR and 7-AAD (BD Biosciences Pharmingen™, San Diego, CA, USA).
Other mean cytokine and surface marker expression in UC E. coli-unladen macrophages varied from CD as follows: IL-6 & IL-8 not significantly different to CD E. coli-laden macrophages; iNOS & CD163, intermediate between CD E. coli-laden and unladen; and COX2 not significantly different to healthy controls (IL-23 and IL-10 not measured for UC) (Fig. 3).
Surface marker expression levels were expressed as the geometrical mean fluorescence intensity on monocytes.
These neutrophils showed decreased mean lobe counts and distinct changes in surface marker expression (CD62Ldim/CD64brt).
The change in marker stain intensity was given by the difference between mean stain intensity after surgery (Ma) and mean before (Mb), whereas the change in the number of pixels demonstrating marker expression was given by Pa Pb.
To evaluate the marker expression, we determined the specific fluorescence index (SFI) using the mean fluorescence intensity (MFI).
ROS through Apoptosis signaling stimulates pluripotency marker expression.
FSK effects towards BC marker expression were next studied through immunolocalization.
(a) Clustergrammer was used to semi-automatically identify cell types based on surface marker expression.
