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Alveolar development was evaluated using the mean linear intercept (MLI) and radial alveolar counts (RAC) as previously described (20, 21).
The most popular parameter for assessment of emphysematous alterations, mean linear intercept length, has severe limitations.
The mean linear intercept length depends strongly on the spread in spatial grain size.
(b) Representative H&E-stained histology (original magnification × 10) and the mean linear intercept shows that loss of CD73 worsens alveolar simplification in 70% oxygen-exposed mice.
Hence, there is no general proportionality between the mean linear intercept length and the spatial grain size.
Analysis demonstrated enlarged alveolar spaces with a significant increase in the mean linear intercept and decreased alveoli number with a significant decrease in radial alveolar count in 95% oxygen-exposed animals (n=4 per group).
A general relationship between the mean linear intercept length in polycrystalline materials and the true mean spatial grain size is derived.
Two samples of recrystallized aluminium with equal mean linear intercept length may differ in mean spatial grain radius by a factor of 2.5.
In grain growth experiments, if the mean spatial grain size is estimated by the mean linear intercept length, marginal reductions in the spread in grain size may be interpreted as an increased growth exponent in the grain growth equation.
d Morphometric analysis of the mean linear intercept.
Grain size was determined by mean linear intercept method.
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