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The mean lifetime for AID bound to ssDNA is ∼5 min14.
Time-resolved intensity decays showed a 2.4-fold decrease in mean lifetime for GlnBP S179C-acrylodan upon the addition of glutamine, indicating the possibility of a lifetime-based assay.
The technical details of calculating the mean lifetime for each Trp are described in Supporting Information.
As a way of initially visualising the data, we fitted a single-exponential decay model to all pixels acquired from each well and then calculated the mean lifetime for that well (see Figure 1 b).
Two-component exponential fitting of τ3 D (18) D e n s i t y = F e − τ t 1 + (1 − F ) e − τ t 2, t1, t2 the mean lifetime for each component.
Similar(54)
The short mean lifetimes for Trp68 and Trp156 are consistent with their very low quantum yields computed by QM-MM methods developed by Callis et al. (18, 24− 24−.
Frank, C. Wissel, A formula for the mean lifetime of metapopulations in heterogeneous landscapes, Am.
The latter was important for calculating the mean lifetime of a state.
Calculation for CH2Cl2 based on a parameterized global mean lifetime of 0.43 years.
Dwell time for L dsDNA = 60 bp represents the mean lifetime of all binding events.
Mean lifetime costs for women was €111,935.96, a third more than for men, at €81,565.67.
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