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The cellular mean lifetime distribution of the three layers is plotted in Figure 3 (g).
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Mean lifetime distributions were calculated from images acquired at 20 μm, 40 μm, and 60 μm depth from skin surface, approximately corresponding to granular, spinous, and basal layer, respectively– 3(f)).
For reaction networks showing non-negligible frequencies, calculating lifetimes as the mean of the lifetime distribution could be considered.
The error bars on the plots of mean lifetime (right-hand plot in Figure 7 b) are the standard deviation of the lifetime distribution, and so indicate both the variation in lifetime due to photon statistics as well as spatial variation in lifetime across each image.
The mean lifetime of individual molecules was 40 ms (1.33 frame) with exponential distribution (lambda = 0.7), which was determined from real recordings of red EGFP single molecules (data not shown).
One can characterize a lifetime distribution through three functions: reliability function, failure rate function, and mean residual life [20].
(f ) Mean lifetime of ATPγS-induced pauses calculated from single-exponential fits to the pause duration distribution at 3 mM [ATP] and 1 mM [ATPγS].
Both their distributions are fairly narrow with FWHM of around 10% of the mean lifetime (610 ps and 1.97 ns for HCV and HCMV, respectively).
Right panel related distribution of lifetime values in the mask, showing a unimodal behaviour with a mean lifetime of 121.3 µs (6.6 µs interquartile range).
Variations of free volumes of cross-linked heat shrink low density polyethylene, prepared by the electron beam irradiation, in terms of temperature are studied through the analysis of positron annihilation parameters, such as S parameter, mean lifetime, lifetime and intensity of pick-off reaction of ortho-positronium (o-Ps), and a size distribution of free volume.
(Right panel, bottom) The distribution of lifetime values in the cartonage in pixels (counts) vs. lifetime, showing a unimodal behaviour with a mean lifetime of 119.3 μs (7.7 μs of interquartile range).
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