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The mean lethal dose (LD50) of Garcinia kola (root bark, stem bark and seed) biflavonoid fractions were determined by a method described by Lorke [13].
Toxicity was expressed as mean lethal dose (LD50) values.
The mean lethal dose (LD50) in mice is approximately 1000 fold lower by injection or inhalation than by oral administration [5].
For the determination of mean lethal dose (LD50), groups of 10 mice were used at each dosage level and 4 or 5 dosage levels were tested per experiment.
Three weeks later, the immunized mice received a lethal challenge with one hundred times the mean lethal dose (100 LD50) of influenza A/PR/8/34 virus (Figure 3A).
Mean lethal time (LT50) and (LT90) values were calculated by fitting the data to the Gompertz distribution model using GraphPad Prism v. 5. Mean lethal dose (LC50) and (LC90) values were calculated using the non-linear regression function of GraphPad Prism and compared using 1-way ANOVA with Tukey's multiple comparisons post-test.
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In clonogenic survival curves of all types of cells, mean lethal doses (Do) of DWA2114R were an order of magnitude greater than those of CDDP.
Mice were challenged with 3 mean lethal doses (LD50) of A/PR8 virus given in 30 μL of saline to the nostrils of sedated mice.
Fatalities have been reported after ingestion of 0.5 g by an adult, with a mean lethal adult dose of 1 4 g [ 1].
The results obtained in our study from three new GBM cell cultures yielded the mean lethal (nonproliferating) dose D0 in the order of 6 8 Gy.
That means a lethal dose would be achieved by eating about 2.5 litres of the poisoned cereal.
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