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For each gene, mean intron length was determined based on the transcript with the largest number of exons among transcripts with FPKM > 1.
Indeed, noisy splicing increased with mean intron length (Spearman's ρ = 0.33, Student's p < 2.8 × 10−231), and decreased with gene expression levels (Spearman's ρ = −0.23, Student's p < 4.6 × 10−109).
First, CDSs were separated into different classes - based on how many introns they contained - and mean intron length was calculated at each intron position within each class (with a requirement of at least 100 introns in each position).
The mean intron length in three-spined stickleback is clearly greater than that in the pufferfish.
Mean intron length is 134 nt, and median intron length is 89 nt (Additional file 4).
Dictyostelium purpureum has a longer mean intron length because of its small proportion of extraordinarily large introns.
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Mean intron lengths were compared by use of a Z-test.
The gene models for loblolly pine have the highest median and mean intron lengths of 24 fully sequenced plant genomes.
In the green algae Chlamydomonas reinhardtii and Ostreococcus tauri, for example, mean intron lengths are 373 and 103 bp, respectively [ 28, 29].
Mean intron lengths of these organisms significantly correlate with their genome length and a noteworthy correlation can be found considering only Vertebrata (Taxonomy ID: 7742, Supplementary Figs S3 and S4).
64% of TDP-1 peaks were contained in introns that were 32-fold longer than the average intron length (mean size of bound intron: 2,120 bp versus 65 bp genome average).
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