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Top, mean intensity of fluorescence (MFI) of cells.
c, Mean intensity of p21 in non-targeting or PARP1-knockdown HeLa cells.
The mean intensity of all cells at each timepoint is shown, and error bars represent SD.
The mean intensity of the green channels in the nuclear region (DAPI positive) was quantified by LAS X (Leica).
Indicated cells were treated as in c. e, Mean intensity of PAR and PARP1 in non-targeting or PARP1-knockdown HeLa cells.
b) Raw STED images from panel a were subjected to background subtraction (mean intensity of all pixels in the image) followed by a Gaussian blur (2 pixel size).
The mean intensity of EGFP was quantified by drawing a free-hand region of interest (ROI) around the cell soma using ZEN 2012 SP1 software.
Mean intensity of pMOH was 2.95.
Maximum and mean intensity of reflectance were higher for all agents compared with controls (P < 0.05).
Reported mean intensity of migraine was 2.6 (scale 1 3).
Reported mean intensity of migraine attacks was 2.7, representing severe pain.
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