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At day 14, there was a significant difference in mean intensity between the two groups (p < 0.05) (Fig. 5b).
Interestingly, there were more nuclei with strong pMAPK signal in γ KCs (VT44966) compared with α'/β' KCs (VT57244) (Fig. 1C), despite that there were no significant differences of nuclear pMAPK mean intensity between these two types of KCs (Fig. 1B).
Due to the difference in mean intensity between the images, each FFT power spectrum was normalized to unit volume.
In order to generate the maps, the mean intensity between the IS and RPE layer was plotted into a 2D map.
Thus, it was possible to quantitatively compare fluorescence mean intensity between YFP and eGFP (Fig. 4C), and between Cherry and Apple (Fig. 4D).
The disparity of the mean intensity between the two cell types could be mainly caused by the inherent nature of these cell types.
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Fold change, the ratio of normalized mean intensities between two conditions, was also used to measure the differential expression of miRNA.
We, therefore, performed ten tests, five tests to test for differences in prevalence between species in five species pairs and five tests to test for differences in mean intensities between species in the five species pairs.
A mean value of above 300 was established by visual confirmation as positive staining, and asymmetric divisions were scored when one daughter cell has a mean pixel intensity of above 300 and the other was 300 or below, and at the same time the difference in mean intensities between the daughter cells was at least 25%.
For our study, we calculated the mean intensity ratio between SP and IZ to demonstrate that these zones become iso-intense, on average, around 26 GW on typical clinically acquired T2-W images and therefore segmentation must be limited to earlier gestational ages.
Additionally, there are mean intensity differences between the center part of the particle (thickest region) and the regions closed to the surfaces in projection (thinnest regions).
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