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Genes were grouped according to their mean intensity (μint).
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We used six biological replicates in order to draw significant conclusions.[ 27] For each gene we calculated the mean signal intensity (μint) and standard deviation of the six biological replicates.
The mean expression intensities (μint) ranged from 73too 690,113 (arbitrary units), (see Additional file 1: Expression level and interindividual variation in all genes on the custom microarray).
Here we measured the standard deviation of pixel intensity and the mean intensity within each cell.
The standard deviation of pixel intensity and mean intensity were measured for each segmented cell.
Top, mean intensity of fluorescence (MFI) of cells.
Mean intensities were normalized to the alpha-Tubulin loading control47 and fold increase in mean intensity was quantified (right plot).
First, all images were calibrated to have the same mean fluorescent intensity based on the overall mean intensity for the whole stack.
Endosomal enrichment was defined as the ratio of the mean intensity in the ROI divided by the mean intensity in the whole cell.
c, Mean intensity of p21 in non-targeting or PARP1-knockdown HeLa cells.
Each channel was converted into the eight-bit grayscale and the mean intensity was calculated.
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