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Top, mean intensity of fluorescence (MFI) of cells.
Endosomal enrichment was defined as the ratio of the mean intensity in the ROI divided by the mean intensity in the whole cell.
Here we measured the standard deviation of pixel intensity and the mean intensity within each cell.
c, Mean intensity of p21 in non-targeting or PARP1-knockdown HeLa cells.
Each channel was converted into the eight-bit grayscale and the mean intensity was calculated.
The solar activity record indicates a mean intensity comparable to that of the mid-20th century.
The standard deviation of pixel intensity and mean intensity were measured for each segmented cell.
The mean intensity of all cells at each timepoint is shown, and error bars represent SD.
Mean intensities were normalized to the alpha-Tubulin loading control47 and fold increase in mean intensity was quantified (right plot).
The mean intensity of the green channels in the nuclear region (DAPI positive) was quantified by LAS X (Leica).
Bot fly prevalence was 28.9% while mean intensity was 5.0 larvae per infected chick.
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