Sentence examples for mean insert sizes from inspiring English sources

Subsequent analysis of paired-end data showed that the paired-end libraries had a very consistent insert size between pools with mean insert sizes ranging from 3,132 to 3,164 bp across the six pools (Fig. 2b).

Unlike other plant and animal species for which we typically obtain clones with mean insert sizes in excess of 100 kb with only modest optimization, application of standard BAC library construction protocols resulted in LP clones with mean insert sizes <75 kb.

Size selection resulted in libraries with mean insert sizes (excluding sequencing adapters) of 250 450 base pairs (bp).

The mean insert sizes and standard deviations were calculated in silico using Picard Tools v1.98 from 1,000,000 reads.

The cDNA mean insert sizes of the libraries have been estimated on 50 individual clones by PCR using T3 and T7 as primers flanking the inserts.

The points outside the confidence strip may represent read-pairs with somewhat larger deviations from the mean insert sizes or chimeric read-pairs.

Assuming the normality of insert size distribution of aligned reads, we generally use 2 to 3 SD from the mean insert size to detect potential deletions and insertions.

However, with the addition of the pre-electrophoresis step, mean insert size was increased to 110 kb for plates 2651-4752.

Many of these steps led to minor increases in mean insert size, but ultimately were not sufficient to provide mean insert lengths ≥100 kb.

A mean library fragment size of 200 250 bp (mean insert size of 100 150 bp) was achieved by gel purification relative to standard sized markers.

The breakthrough that permitted realization of the >100 kb mean insert size goal came with the discovery and adoption of the "pre-electrophoresis" procedure of Osoegawa et al. [15].