Assuming the normality of insert size distribution of aligned reads, we generally use 2 to 3 SD from the mean insert size to detect potential deletions and insertions.
However, with the addition of the pre-electrophoresis step, mean insert size was increased to 110 kb for plates 2651-4752.
Many of these steps led to minor increases in mean insert size, but ultimately were not sufficient to provide mean insert lengths ≥100 kb.
A mean library fragment size of 200 250 bp (mean insert size of 100 150 bp) was achieved by gel purification relative to standard sized markers.
In brief, the BAC library (as of 2/18/2008) contains a total of 1,612,800 clones with a mean insert size of 94 kb and represents 7× coverage of the P. taeda genome.
The LP BAC library consists of 1,824,768 individually-archived clones making it the largest single BAC library constructed to date, has a mean insert size of 96 kb, and affords 7.6X coverage of the 21.7 Gb LP genome.
The mean insert size was around 250 300 bp.
Mean insert size was 3 kbp and std 20 bp.
The mean insert size of the pairs was 353.
BAC DNA were purified from two Nile tilapia BAC libraries, T3 library (mean insert size 145 kb) and T4 library (mean insert size 194 kb) [ 59].
