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The sensitizer enhancement ratio (SER) in SKBR3 cells was defined as SER = mean inactivation dose (radiotherapy)/mean inactivation dose (plasmid +radiotherapy), as previously described.
The radiation enhancement factor was calculated as the ratio of the mean inactivation dose of control cells divided by the mean inactivation dose of 14-3-3σ 14-3-3σ 14-3-3σ and knockdown cells as previously described [ 18].
The mean inactivation dose (equal to the area under the survival curve) was also calculated according to the method of Fertil (Fertil et al, 1984).
The radiation enhancement ratio (ER) was calculated as the ratio of the mean inactivation dose under control conditions divided by the mean inactivation dose of experimental conditions (that is, another cell line, or siRNA or drug treatment), as described previously (Morgan et al, 2008).
The parameters included D0, D q, and N. D0 is the dose at which 63% of the cells are killed or 37% of the cells survive, and it represents the mean lethal dose or the mean inactivation dose.
The following parameters were calculated: IC50 (drug concentration causing 50% growth inhibition); ID50 (radiation dose causing 50% growth inhibition); SF2 (surviving fraction at 2 Gy); and MID (mean inactivation dose, calculated by numeric integration of the linear-quadratic curve [ 36]).
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The sensitizer enhancement ratio was calculated as the ratio of the mean inactivation doses, defined as the dose at which there is 37% survival, with and without GPMs.
A panel of eight human pancreatic tumour cell lines displayed high intrinsic radioresistance, with mean inactivation doses between 2.4 and 6.5 Gy, similar to those reported for melanoma and glioblastoma.
Inactivation was normalized to the mean inactivation in the control period.
Mean dose and dose volume histogram is a most convenient tool for incorporating such constraints.
For daily exposures performed on 24 days in 2002 2003, the values of the observed spore inactivation doses (SID) and the calculated ones were closely correlated with a mean ratio (observed SID/calculated SID) of 0.7 ± 0.3, while the values of the observed SID were regressed to the erythemal doses (MED) by a power function with a correlation coefficient r2 ∼ 0.9.
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