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The mean grey value of the ROI was determined with ImageJ.
All images in the study were first equalized in luminance (mean grey value = 128) and in RMS contrast (standard deviation of 20.4).
The integrated density mean grey value of the band was analyzed under ImageJ software and the corresponding relative expression ratio was calculated.
The mean grey value of the implant was measured with ImagePro Plus 4.0 (Media Cybernetics) on 1 mm of the implant, measured from the end implanted inside the larva.
The mean grey value in CSF was higher in both types of hydrocephalus; obstructive (14.7% mean grey value) and communicating hydrocephalus (38.3% mean grey value) when compared to the control CSF (6.9% mean grey value).
We analysed the mean grey value of each image.
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(P ) Western-blot quantification (mean grey values).
Filopodia were identified as needle-like, phalloidin-stained surface protrusions; filopodia numbers reflect the total amount of filopodia per neuron; length was measured via ImageJ from the tip to the point at their base where filopodia dilate; protein levels were measured in ImageJ and represent the mean grey values at sites of protein accumulations.
Depicted values represent quantification of mean grey values of corresponding bands.
The DR5rev:mRFP signal intensity can be easily estimated by measuring the mean grey values.
The changes in the mean grey values over time were not statistically significant (p=0.4941) (figure 2).
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