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In the apical coils the mean grey scale levels were 45.9±3.0 (n = 11) in the +/av6J heterozygotes versus 34.9±2.8 (n = 6) in the av6J/av6J mutants, and in the basal coils the mean grey scale levels were 87.4±4.6 (n = 11) in the +/av6J heterozygotes versus 69.6±7.1 (n = 6) in the av6J/av6J mutants.
The mean grey scale intensity of domain images is plotted with respect to its corresponding time to obtain the relaxation curve.
The mean grey scale pixel intensities of GFP within PDF neurons were calculated by individual ROI circular masks outlined by the PDF-positive cell body (GFPpdf).
A vector with the spectral information in the whole [450, 990]nm interval was created with the mean grey scale value of a Region Of Interest (ROI), normalized between 0 and 1.
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This program can calculate mean grey-scale value within the cross sectional area of a single muscle fiber.
To obtain an image of chromatin density fluctuations, the mean grey-scale value was subtracted from the image.
This means that the blob analysis may quantitatively separate scans of different muscle composition, even though the mean grey-scale intensity is the same.
The image analyses supplemented each other and gave a more complete description of the tissue composition in the muscle than the mean grey-scale value alone.
Even if diseased muscles typically look diffused or unstructured, a well-trained muscle could be characterized by almost the same mean grey-scale value because of a distinct intramuscular tendon, e.g. in the supraspinatus muscle.
The mean grey-scale intensity was higher in the vastus lateralis muscle (p < 0.05) than in the supraspinatus muscle (average value of middle measuring site 51.4 compared to 35.0).
The threshold values were defined on the basis of the grey-scale values in the 25%75%% quartiles interval for the mean grey-scale intensity for the healthy group.
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CEO of Professional Science Editing for Scientists @ prosciediting.com