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For statistical pattern recognition, the following parameters were used: form factor, mean grey level, signal-to-noise ratio, mean gradient and correlation from the co-occurrence matrix.
Fig. 4 Correlation of BLI light signal (mean grey level intensity) and tumour volume in the para-pancreatic area determined post mortem.
Indeed, in a double classification from signal and global image, it is appropriated to classify the signal (sampled amplitude of signal which is more local than global) using neural local approximators (RBF), while, it is appropriated to classify the global image (area mean grey level of global image which is more global than local) using neural global approximators (MLP).
The mean grey level of the 64 data lines at depth z was then calculated by.
The fluorescence was quantified by using the measure function in the program to give the mean grey level of a designated area.
In order to minimize possible variation due to differences in focal depth in different subjects, the mean grey level f z) mean was normalized to the maximum mean f z m ) within the ROI as f z) mean / f z m ).
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For the qualitative mapping, signal intensities were grouped (- 0-10; +10-20; ++20-60; +++ >60 mean grey levels).
Ovariectomy induced a consistent, although non-significant, decrease in mean grey-level intensity in calvariae without PE particles as compared to normal controls.
Specifically, mean grey-level values were 117.6 ± 7.7 (range, 107 to 48), 115 ± 2 (range, 107 to 120) and 110 ± 0.3 (range, 109 to 111), in normal controls, sham-OVX mice and OVX mice, respectively.
By using the law of total probability, P g, G| x = k) can be used to calculate as follows: (10) which, using assumption 1, can be written as: (11) It also assumes that f(g| x = k) follows a normal distribution with parameters μ k, the mean grey-level for class k, and σ k, the standard deviation of the gray-scale values for class or label k.
Signal intensity was quantified as the mean of grey level per second of time exposure within a region of interest (ROI) prescribed over the muscle using the Simple PCI software.
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